BY SABRINA EDWARDS ’18
Q: Where did you do research this summer?
A: I was here at Mount Holyoke in Professor McMenimen’s lab.
Q: What was your project?
A: I was assigned a new project with my lab partner during the spring semester, but my lab partner had another internship so she wasn’t here for the summer, so I was alone in the lab. I created a TAG point mutation in the n-terminus region using PCR of a small heat shock protein, specifically HSP27. Traditionally, the translational machinery would stop translation at the stop codon, and produce a truncated version of the protein. In my research, I transformed an orthogonal translational machinery and with the addition of unnatural amino acids (UAA), we are hoping to see the machinery charge the UAA at the stop codon, and continue translating to produce a full length protein.